Suddenly I have the prohibited sign? (Sierra)

I've had my Hackintosh setup for about 9 months, I've got Sierra on it but for some reason today is stopped working. I keep getting the prohibited sign the second after I see the apple logo pop up.

I've never really had boot issues ever since I got it set up 100%. I've re-downloaded Clover on a USB thinking that was the issue, I can't seem to find issues relating to the prohibited sign after you've been using the OS.

Any help would be appreciated!

submitted by /u/YaloSwog
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